The Fundamentals of Entrepreneurial Acculturation course introduces students with the concepts and theories of entrepreneurship, such as creativity and innovation, new business idea generation, and new enterprise management. Experiential entrepreneurial learning is the emphasis of this course. The course develops student’s entrepreneurial mindsets, entrepreneurial skills, essential characteristics, and necessary competency through the whole process from problem solving, idea generation, and feasibility analysis to projects/activities implementation. Upon completion of this course, students may possess entrepreneurial characteristics and necessary skills to start up a new business in line with Industry 4.0 agenda.